Video assistant referee (VAR) technology will be tested across multiple matches in the Premier League for the firmatches on Saturday.
In April, Premier League clubs voted against introducing VAR for the 2018-19 campaign.
After VAR tests at individual fixtures last season, the Premier League now wants to see how the system copes with several games at the same time.
The trial, across all five 3pm kick-offs, will be at the central VAR hub.
There will be no communication with anyone at the grounds at which the games are taking place.
The trial limits the use of VAR to goals, penalty calls, direct red cards and cases of potential mistaken identity.